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Download Anti-Porn Download Any Video Converter Ultimate 6. Download Advanced SystemCare Pro 9. Download IObit Uninstaller Pro 6. Download iExplorer 3. Download Wireless Network Watcher 2. Download Total Uninstall Professional 6. Astringent taste is due to the presence of polyphenols 12 , and the scientific research on PJ demonstrating pomegranate tannins obtained from peels without the sweetness health benefits has largely been carried out with juice squeezed of the sugars found in whole pomegranate.

Adulteration methods from whole fruit Figure 2. A Typical anthocyanin chromatogram illustrating the six anthocyanins found in authentic PJ sample S, peak 1, delphinidin-3,5 diglucoside; peak 2, cyanidin-3,5-diglucoside; peak 3, delphinidinglucoside; peak 4, pelargonidin-3,5-diglucoside; peak 5, cyanidinglucoside; peak 6, pelargonidin- 3-glucoside. To establish a reference point for these studies, other The present studies employed a panel of different analytical databases from The European Fruit Juice Association AIJN techniques to study pomegranate fruit purchased from the 16 and a database from Krueger Food Laboratories 17 were market and 23 different commercial juices from local markets used to establish initial parameters of authenticity.

Then our claiming to be authentic pomegranate juice. Complete analyses, reported in the present paper, developed new param- chemical profiles including pomegranate polyphenols, sugar eters for mannitol and tartaric content of PJ and stable isotope profiles, organic acids, amino acids, and potassium were ratio analysis SIRA to detect adulteration with C4 sugars. The initial pomegranate juice Figure 1. Cyanidinglucoside and punicalagin A and B standards and detected at nm.

A linear gradient of two component solvents, were purchased from Chromadex Irvine, CA. Sucrose, glucose, acetonitrile solvent A and 0. Ellagic acid, citric acid, D-malic acid, L-malic acid, oxalic acid, juice. All juice samples were purchased respectively. The numbers of lots of the same juice ranged Sugar Analysis. The and a RI detector module controlled by Empower 2 software was juice samples were inventoried, shaken well, transferred into five applied for the sugar content analysis.

Pomegranate Polyphenol Analysis. Pomegranate polyphenols were detector. For anthocyanin of the components of juice. The column Table 4. Specifications IMAS for Commercial Pomegranate Juice For the detection of added cane sugar and high-fructose corn syrup, stable isotope ratio mass spectrometry was used to accurately determine attribute method IMAS criterion carbon enrichment.

Plants fix CO2 by one of two common pathways: the Calvin delphinidin-3,5-diglucoside delphinidinglucoside cycle pathway C3 or the Hatch-Slack pathway C4. C3 photosyn- cyanidin-3,5-diglucoside thesis has a very large isotope effect associated with carbon dioxide cyanidinglucoside fixation, whereas C4 photosynthesis involves only a small degree of two minor peaks carbon isotope fractionation.

Empower 2 software was applied for the organic acid content analysis. Low potassium should solvents, acetonitrile solvent A and 0. Samples were analyzed for proline by photometric g The mannitol, sorbitol, and sucrose levels and glucose detection at nm after reaction with ninhydrin AOAC method The ratio of Potassium.

Potassium was measured with a flame photometer using glucose to fructose varied in a narrow range of 0. The PJ samples were diluted with water to below the authentic PJ. In addition, sucrose was not detected in 10 ppm prior to analysis. As shown in Figure 3 there are Chemical determinations of anthocyanin, ellagitannin, sugar characteristic ratios of the glucose and fructose peaks profiles, organic and amino acids, and potassium contents were demonstrated in the chromatogram of sample O Figure 3A carried out in 45 commercial juice specimens from 23 different and an abnormal ratio of these sugars together with the manufacturers purchased at local grocery stores see Tables presence of sucrose in sample I Figure 3C and with the Whereas concentrations of ellagitannins varied in com- presence of sucrose in sample G Figure 3B on the HPLC mercial PJ samples tested, the characteristic ellagitannins were profile.

Charac- fruit sugars in multiple samples. As characteristic polyphenol profile. However, in several of sample G. This U. The latter may be an analytical error, and more lots would be tested for IMAS specification in such a case.

Table 6. The IMAS was then configured to enable pomegranates and to consumers who purchase this product for detection of each of these methods through measurement of its health value but are cheated through substitution of non- polyphenols, sugar profile, organic and amino acids, and pomegranate juice ingredients. Table 5. Sucrose, invert sugars, and high-fructose syrups such as high- We concluded that authentic commercial pomegranate juice fructose corn syrup can be detected by using SIRA.

The made from whole fruit is characterized by consistent composi- photosynthetic process in fruits is different than that in cane tion regardless of variety or geographic origin. There are always sugar or corn, resulting in their having a different preference six characteristic anthocyanins present, but the concentrations for carbon compared to carbon in the process of may vary see Figure 1.

Punicalagins are always present, but photosynthesis. Corn and sugar cane belong to the less common do not prove authenticity, necessitating further chemical char- C4 photosynthetic category, whereas most other commercially acterization.

There is a characteristic multidimensional sugar important plants belong to the C3 category This observa- profile based on measurement of mannitol, glucose, and fructose. This procedure has since been collabora- of common adulteration methods. Subsequent work has extended these findings to evaluations The establishment of the IMAS for PJ based on the analyses of grape juice 23 , cranberry juice 24 , raspberry juice 25 , in the present study is a model that can be used for other pineapple juice 26 , and other fruits 27 , including pomegranate examples of fruit juice adulteration.

The current federal guideline SIRA can accurately detect adulteration with cane or corn- for PJ authenticity is based only on the minimum quality derived sugars. Both sugar profile and organic acids can be used to detect This criterion assumes that the starting concentrate is authentic adulteration with grape or apple sugars.

The total level of acids PJ and merely establishes a degree of dilution that is permissible. Total titratable acid 1 Gil, M.

Additionally, malic Changes in pomegranate juice pigmentation during ripening. Food Agric. Contrary 2 Hernandez, F. Food Res. California 3 Miguel, G. Anthocyanins charac- found in pomegranate. We conclude that pomegranates do not terization of 15 Iranian pomegranate Punica granatum L. The detection of tartaric acid indicates adulteration with grape Eur. Sucrose, sorbitol, and maltose were not found in 5 Gil, M. Antioxidant activity of pomegranate juice adulteration.

Previous studies of 40 Spanish pomegranate and its relationship with phenolic composition and processing. In several other studies, no sucrose was R. Antioxidant activity, polyphenol content, and related com- detected in fresh pomegranate fruit The detection of pounds in different fruit juices and homogenates prepared from sucrose indicates that adulteration with cane sugar is likely. Beet or cane total invert sugars 7 Seeram, N. High-fructose syrups are produced by the enzymatic hydrolysis 8 Guo, C.

Pomegranate juice is potentially better than apple juice in improving antioxidant of corn, potato, rice, or wheat starch. The resulting glucose syrup function in elderly subjects.

D-Sorbitol indicates addition of synthetic sweetener and 10 Jimenez, D. M; Ramazanov, A. A new method of standartization of health- should not be detected in PJ.

Generally, investigators find zero promoting pomegranate fruit Punica granatum extract. Georgian to trace levels of sorbitol in the pomegranate D-Sorbitol Med. News , 70— Mannitol is found is significant amounts in.

Therefore, most of the adulteration tactics Y. In vitro antiproliferative, apoptotic can be detected by examining the sugar profile. Pomegranate juice is a , 16, — The finding 13 Pantuck, A. Potassium typically is the main mineral in pomegranate Elashoff, R. Like most other fruit varieties, fresh pomegranates contain Phase II study of pomegranate juice for men with rising prostate- only traces of sodium by nature.

Cancer Res. The history of juice adulteration for profit is extensive, and 14 Sumner, M. Effects of research conducted with genuine PJ while denying the public pomegranate Juice consumption on myocardial perfusion in the very phytochemicals that account for the unique health patients with coronary heart disease.

Furthermore, use of nonauthentic PJ in research —

 

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